Development of an Automated Handheld Immunoaffinity Fluorometric Biosensor
نویسندگان
چکیده
new immunoaffinity fluorometric biosensor has been developed for detecting and quantifying aflatoxins, a family of potent fungi-produced carcinogens that are commonly found in a variety of agricultural products. They have also been cited as biological agents under weapons development. The handheld, self-contained biosensor is fully automatic, highly sensitive, quick, quantitative, and requires no special storage. Concentrations from 0.1 to 50 parts per billion can be determined in less than 2 min with a 1-mL sample volume. The device operates on the principles of immunoaffinity for specificity and fluorescence for a quantitative assay. The analytic procedure is flexible so that other chemical and biological analytes can be detected with minor modifications to the current device. Advances in electro-optical components, electronics, and miniaturized fluidics were combined to produce this reliable, small, and versatile instrument. (
منابع مشابه
A High Throughput Biochemical Fluorometric Method for Measuring Lipid Peroxidation in HDL
Current cell-based assays for determining the functional properties of high-density lipoproteins (HDL) have limitations. We report here the development of a new, robust fluorometric cell-free biochemical assay that measures HDL lipid peroxidation (HDLox) based on the oxidation of the fluorochrome Amplex Red. HDLox correlated with previously validated cell-based (r = 0.47, p<0.001) and cell-free...
متن کاملDetermination of ochratoxin A in grapes, dried vine fruits, and winery byproducts by high-performance liquid chromatography with fluorometric detection (HPLC-FLD) and immunoaffinity cleanup.
A liquid chromatographic method for the determination of ochratoxin A in grapes, dried vine fruits, and winery byproducts was developed. A mixture of either acetonitrile/water or acetonitrile/water/methanol was used as an extraction solvent mixture. After immunoaffinity column cleanup, the final extract was analyzed by high-performance liquid chromatography (HPLC) with a fluorometric detector (...
متن کاملDesign and Development of Microcontroller Based Auto-Flow Assembly for Biosensor Application
An automated flow assembly for a biosensor has been developed in this paper. In recent years there has been a growing interest in the field of in-vivo biosensors for continuous monitoring. For this continuous monitoring of biological interactions, a need for the development of auto-flow assembly arises. The automated flow assembly inherently coupled to a biosensor, forms a Flow Injection Analys...
متن کاملMicrofluidic biosensor for β-Hydroxybutyrate (βHBA) determination of subclinical ketosis diagnosis
BACKGROUND Determination of β-hydroxybutyrate (βHBA) is a gold standard for diagnosis of Subclinical Ketosis (SCK), a common disease in dairy cows that causes significant economic loss. Early detection of SCK can help reduce the risk of the disease progressing into clinical stage, thus minimizing economic losses on dairy cattle. Conventional laboratory methods are time consuming and labor-inten...
متن کاملDetermination of ochratoxin A in wine and beer by immunoaffinity column cleanup and liquid chromatographic analysis with fluorometric detection: collaborative study.
The accuracy, repeatability, and reproducibility characteristics of a liquid chromatographic method for the determination of ochratoxin A (OTA) in white wine, red wine, and beer were established in a collaborative study involving 18 laboratories in 10 countries. Blind duplicates of blank, spiked, and naturally contaminated materials at levels ranging from < or =0.01 to 3.00 ng/mL were analyzed....
متن کامل